Pathology
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Atlas of dermatopathology
Josef Feit, Hana Jedličková, Günter Burg, Luděk Matyska, Spasoje Radovanovic et al.
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+ Introduction
+ Literature
+ Support
+ Skin biopsy
+ Non-tumorous skin diseases
+ Skin tumors
+ Cysts of the skin and subcutis
+ Soft tissue lesions
+ Some lesions of the oral cavity
+ Dermatovenerology
+ Collection of histological slides by prof. Günter Burg
+ Collection of histological slides by prof. Werner Kempf
+ Collection of histological slides of dr. Ram Chandra Adhikari
Skin biopsy
Introduction

Specimens of the skin are taken for diagnostic as well as cosmetic reasons. Most commonly used method is transmitted light microscopy. The tissue is fixed, cut, mounted on a glas, stained and examined. The most common staining is hematoxylin-eosin; but other stains (eg. PAS, alcian blue, silver nitrate impregnation, trichromes, Giemsa, toluidine blue and alcian blue) are often used.

Sometimes, however, formalin fixation prevents detection of some tissue structures (enzymes, antibodies, some antigens) and requires using unfixed, frozen tissue. For staining neutral fat it is necessary to avoid paraffin processing.